在老鼠的蜕膜细胞雌激素受体α和β:特异性表达和微分调节类固醇激素和催乳激素。
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Tessier C, Deb年代,Prigent-Tessier Ferguson-Gottschall年代,Gibori GB,萧若元RP, Gibori G
在老鼠的蜕膜细胞雌激素受体α和β:特异性表达和微分调节类固醇激素和催乳激素。
内分泌学。2000年10月,141 (10):3842 - 51。doi: 10.1210 / endo.141.10.7734。
- PubMed ID
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11014241 (在PubMed]
- 文摘
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雌二醇是扮演着一个重要的角色在大鼠子宫基质细胞的生长和分化成蜕膜细胞。特别是,这种激素和孕激素是必要的胚泡植入和随后的decidualization老鼠。虽然绑定实验证明estrogen-binding网站的存在,没有证据表明存在是否大鼠蜕膜表达雌激素受体(ER)的两个亚型,α和β。在这个调查中,我们分析了蜕膜的表达ERalpha ERbeta,研究他们的监管,PRL和类固醇激素检查蜕膜ERp的孕激素受体转导雌二醇信号。免疫细胞化学、rt - pcr和北部污点分析表明,在蜕膜ER物种都coexpressed假孕。优先发现有趣的是,这些基因在细胞群本地化antimesometrial的子宫胚泡植入。使用蜕膜细胞主要文化从pseudopregnant老鼠和decidua-derived细胞系(GG-AD),我们将展示一个微分调节ERalpha和ERbeta PRL和卵巢类固醇激素。而PRL、雌二醇和孕酮都增加ERbeta信使核糖核酸(mRNA)剂量依赖性的方式表达,只有PRL上调的mRNA水平的时代。雌二醇对ERalpha表达式没有影响,而孕激素显著降低其mRNA水平。孕激素,有趣的是,这让光杆载荷信号的能力PRL-regulated基因在乳腺派生细胞,预防PRL刺激蜕膜ERalpha ERbeta表达式没有协同效应。 The use of GG-AD cells, which express only ERbeta, allowed us to demonstrate that this receptor subtype is functional and transduces estradiol signal to the progesterone receptor. In summary, the results of this investigation have revealed that ERbeta is expressed in addition to ERalpha in the rat decidua, and that the expression of both ERs are cell specific and differentially regulated by PRL and steroids. One salient finding of this investigation is that progesterone down-regulates ERalpha, but concomitantly increases the expression of a functional ERbeta that mediates estradiol up-regulation of the decidual progesterone receptor.